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If you're a student and not graduate, you may be able to take advantage of the flexible schedule when you travel. Many campuses have a transportation program, although you could also apply directly to an institution with a bus or cab driver program.
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Muscle mass
Muscle mass was assessed using magnetic resonance imaging, with a Seiko SP-8i imaging system, using an arm-anchored 7T high field coil. For determination of muscle volume, we used magnetic resonance calorimetry (MRS). Body composition (lean mass, fat and body mass) was determined by dual-energy X-ray absorptiometry.
Procedure
For the VO2max test, subjects began exercise protocol in a seated position, wearing light cotton shirt over the torso. After an initial warm-up period of 30 s, treadmill running (3,200 m at 60% of maximum oxygen consumption) was commenced for 20 min. During treadmill running, an electronic sensor placed on the thigh detected heart rate and oxygen cost (Bod Pod software, GE Healthcare, Boston, MA, USA). After 20 min, the accelerometer began measuring rate of the subjects.
For the bench press test, subjects began by seated on the bench; maximal lifting weight of 90.5 kg for women and 95.2 men was achieved on three occasions (10-20 reps each). Participants then performed 10 max effort repetitions by lifting the weight. They were permitted to rest during a 10-sec recovery period after each maximal lifting effort.
For the leg curl test, subjects first entered the machine on a level surface order dexamethasone over the counter and were instructed that maximal voluntary contraction (MVC) was achieved when the participant raised leg until hip flexed and the knee to 90 degrees. MVC was defined as the point where lower quadriceps mass crossed the knee joint and thigh mass was separated from the knee joint by 7 cm. After resting for 2 seconds, the participant commenced an MVC with the knee flexed. On last repetition of the MVC, participant was instructed to bend at the knee and bring down on contact.
For 1-RM testing, subjects were asked to perform 50 maximal repetitions on the leg press machine without any assistance to increase the generic pharmacy online load 80%, 85%, 75%, and 70%, respectively.
For testing of muscle glycogen, subjects remained seated for 1 min prior to commencement of the test. Subjects then began test by resting for 10 min and performing a warm-up of 10-15 maximal repetitions with a controlled eccentric contraction. Subjects then performed two 10-min bouts of muscle biopsy.
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